Short Communication: Genetic similarity analysis of in vitro cultivated pineapple (Ananas comosus) from Sipahutar, North Sumatra, Indonesia using ISSR markers

Abstract. Harahap F, Nusyirwan N, Afiva A, Prasetya E, Suriani C, Hasibuan RFM, Poerwanto R. 2022. Short Communication: Genetic similarity analysis of in vitro cultivated pineapple (Ananas comosus) from Sipahutar, North Sumatra, Indonesia using ISSR markers. Biodiversitas 23: 5623-5628. Sipahutar Indonesia, is considered difficult to breed, resulting a lack seed availability. However, tissue culture allows the emergence somaclonal variations, especially plantlets undergoing several subculture stages. This study aimed identify variations seedlings generated Inter Simple Sequence Repeat (ISSR) Fifteen accessions were used this consisting grown its original environment, laboratory acclimatized, and cultured. Total 10 primers, which seven primers detected polymorphisms among population. resulted low genetic The diversity plants propagated by had level 75%-94%, showing culture. was divided into two main clusters, namely cluster A consisted 11 B 4 accessions. Cluster separation does not correlate source plant materials studied.